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“国内最新相关科研成果”系列之2018年第一期

GCCC 2018-11-02 21:27:46

DANDAN WANG& XINHUI LI& ZHIYING CHENG& CHUNLIN Long

利用弥勒苣苔的芽进行离体保存和组培快繁

In vitro preservation and micropropagation of Oreocharis mileense(W.T. Wang) M. Möller & A. Weber (Gesneriaceae) through shootorganogenesis

tIn Vitro Cellular & Developmental Biology – Plant

摘要

弥勒苣苔Oreocharis mileense (W.T. Wang) M. Möller & A. Weber是中国特有种，曾被认为已经灭绝，因为自从1906年首次从野外收集到标本后，再也没有在野外被发现过。2006年该物种在中国云南省石林县被重新发现。弥勒苣苔因其狭隘的地理分布范围和极少量的种群数量被认定为极度濒危物种。有关弥勒苣苔有效的离体保存和组培快繁的方法尚未见报道。本研究采用三因素正交实验法[6-苄氨基腺嘌呤(6-benzyladenine, 6-BA)；α-萘乙酸(α-naphthaleneacetic acid), NAA和蔗糖），记录在四个水平上进行的芽引导和增殖。

原文链接：https://link.springer.com/article/10.1007/s11627-018-9941-y

ABSTRACT

Oreocharis mileense (W.T. Wang) M. Möller & A. Weber is endemic to China and was considered to be extinct because it had not been seen in the wild since the first collection in 1906. In 2006, the species was rediscovered in Shilin County, Yunnan Province. Oreocharis mileensewas considered critically endangered for its narrow geographic range and extremely small population. An efficient method to preserve plant germplasm by in vitro culturing of O. mileensehas not been reported. In this study, an orthogonal array with three factors (6-benzyladenine, BA; α-naphthaleneacetic acid, NAA; and sucrose), at four levels was performed, and shoot induction as well as shoot proliferation were recorded. The results were analyzed to determine the most significant components and the optimum combination for micropropagation of O. mileense. The results showed that: (1) organogenesis was easily induced by different combinations of plant-growth regulators and sucrose; (2) NAA and sucrose had the most significant effect on shoot induction and shoot multiplication, and (3) the optimum induction and proliferation media were .5 mg L-1 BA + 0.2 mg L-1 NAA + 30 g L-1 sucrose and 1 mg L-1 BA + 0.1 mg L-1 NAA + 30 g L-1 sucrose, respectively.

Original article link: https://link.springer.com/article/10.1007/s11627-018-9941-y

图1 弥勒苣苔在MS培养基上的再生.

(a) 种子在含有0.5%活性炭的MS培养基上萌发10周. (b) 无菌种子在MS培养基上培养9周后萌发无菌苗.(c) 在含有2 mg L-1 BA和0.1 mg L-1NAA的MS培养基上培养1个月后，从外植体中产生的不定芽. (d)在含有2.0 mg L-1 BA 和 0.1 mg L-1 NAA 的MS培养基上培养14周后从不定芽长出的小植株. (e) 发育良好的幼苗被分离出来，准备移植. (f)将幼苗移栽到含有2:1 (v/v)腐殖质:红壤的土壤混合物的花盆中。比例尺为1cm。

Figure 1. Regeneration of Oreocharis mileensis (W.T. Wang) M. Möller& A. Weber on Murashige and Skoog (MS) medium (Murashige andSkoog 1962). (a) Seed germination on MS medium containing 0.5%activated charcoal after culture for 10 wk. (b) Sterile plantlets derivedfrom aseptic seed germination on MS medium after culture for 9 wk.(c) Adventitious shoots derived from leaf explant on MS medium containing 2 mg L-1 6-benzylaminopurine (BA) and 0.1 mg L-1 α-naphthaleneacetic acid (NAA) after culture for 1 mo. (d) Plantlets developed from adventitious shoots grown on MS medium containing 2.0 mg L-1 BA and 0.1 mg L-1 NAA after culture for 14 wk. (e) Welldeveloped plantlets were detached and ready for transplanting. (f)Plantlets were transplanted to a pot containing a 2:1 (v/v) soil mixture of humus:red soil. Bars represent 1 cm.