重组人白介素-15 (rhIL-15)
重组人白介素-15蛋白是在大肠杆菌中表达的，是一条单链、非糖基化的多肽链，包含114个氨基酸。
Iterleukin-15(IL-15) is a widely expressed cytokine that is structurally and functionally related to IL-2. Mature human IL-15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternate splicing generates isoforms of IL-15 with either a long or short signal peptide(LSP or SSP), and the SSP isoform is retained intracellularly.IL-15 binds with high affinity to IL-15Rα. It binds with lower affinity to a complex of IL-2Rβ and the common gamma chain(γc) which are also subunits of the IL-2 receptor complex. IL-15 associates with IL-15Rα in the endoplasmic reticulum, and this complex is expressed on the cell surface. The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15Rα are coordinately expressed on the surface of one cell and interact with complexes of IL-2Rβ/γc on adjacent cells. This enable cells to respond to IL-15 even if they don’t express in IL-15Rα.Soluble IL-15 binding forms of IL-15Rα can be generated by proteolytic shedding or alternate splicing. These molecules retain the ability to bind tightly to IL-15 and can ether inhibit or augment IL-15 function. Consistent with its shared use of IL-2 receptor subunits,IL-15 induces IL-2-like effects in lymphocyte development and homeostasis. It is particularly important for the maintenance and activation of NK cells and CD8+ memory T cells. IL-15 also exerts pleiotropic effects on other hematopoietic cells and nonimmune cells. Ligation of membraneassociated IL-15/IL-15Rα complexes induces reverse signaling that promotes cellular adhesion,tyrosine phosphorylation of intracellular proteins,and cytokine secretion by the IL-15/IL-15Rα expressing cells.
指标：
外观：白色至类白色冻干粉
气味：无异味
活性：采用CTLL-2细胞株增殖的方法进行测定，比活性³5x108IU/mg
纯度：³97%：
内毒素含量：£1EU/ug，鲎试剂法测定
应用领域：细胞培养，诊断试剂，实验检测等.
